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Tissue Research » Current Abstracts
1.Journal of  Cell and Tissue Research  18(2): 6415-6422 (2018)
Non-target impacts of fipronil bait using morphological alterations in the reproductive system of Blaps polycresta (Coleoptera: tenebrionidae) as a biomonitor
Osman, W.
Department of Zoology, Faculty of Science, Alexandria University, Alexandria 21568, Egypt
E. mail:,
Abstract: Insecticide baits have been successfully used against major groups of urban pests. However, they could affect a wide range of non-target fauna. In Egypt, fipronil bait is a pest control method used for elimination of cockroaches at indoor locations. In the present study, morphological alterations in the reproductive system of both males and females Blaps polycresta (Coleoptera: Tenebrionidae) were used to determine the effects of fipronil bait on beetles as non-target insects. Two groups of insects (control and treated) were used in which, the treated one was exposed to fipronil bait (0.05 %). Treated males showed marked decreases in the size of testes and accessory glands with shrinkage of testicular follicles while treated females exhibited ovarian developmental retardation and ovarian morphological deformations. A highly significant increase was recorded in the number of insects contained abnormalities in both males and females of the treated group as compared with the control one. Mortality was examined in two other groups (control and treated) under the same condition of the morphology test in which, 100 % mortality was achieved after about two weeks for both sexes of the treated group while no mortality was obtained in either sexes of the control one throughout the experiment. The obtained data reinforce the importance of non-target impacts of insecticides and the results support the possible use of morphological alterations in the reproductive system of beetles as a biomonitor of fipronil bait effects.
Key words: Fipronil bait, Coleoptera
2. Journal of  Cell and Tissue Research  18(2): 6423-6434 (2018)
Isolation, screening and characterization of PGPR strains isolated from the Rhizosphere of a xerophyte Euphorbia virosa
 Popat, T. and   Vyas, B.R.M.
Department of Biosciences, Saurashtra University, Rajkot, Gujarat, India. E. mail:,
Abstract: Application of exotic bacterial strains as biofertilizer has always posed a constraint in the success of biofertilizer technology. Only limited number of biofertilizers with applicability in wide range of soils is available in the market. Plant Growth Promoting Rhizobacteria (PGPR) with multiple plant growth promoting traits like phosphate solubilization, production of indole acetic acid, gibberellic acid, 1-aminocyclopropane 1-carboxylic acid deaminase activity and antifungal activities like siderophore, hydrogen cyanide (HCN) and extracellular enzymes production were isolated from the rhizospheric soils of Euphorbia virosa growing in semi arid region of  Gujarat. Three isolates having multiple plant growth promoting activities were selected and identified on the basis of 16S rDNA sequence analysis. The application of PGPR strains in vitro has shown remarkable plant growth promotion and antifungal activity and increased in the growth of five crop plants viz. Pisum sativum (Pea), Arachis hypogaea (Ground nut), Cicer arientinum (Gram), Triticum aestivum (Wheat), Vigna radiata (Mung bean) during pot trials was observed.  Our results demonstrate that microbial inoculation improved growth of crop plants and can lead to increased productivity.
Key words: PGPR strains, Biofertilizer, Phosphate solubilization
3.Journal of  Cell and Tissue Research  18(2): 6435-6440 (2018)
In  vitro  mass  multiplication  of  fig  (Ficus carica l.) through  nodal  segment  explants
 Sen, D.K. and  Patel, R.M.
Department of Fruit Science, ASPEE College of Horticulture and Forestry, Navsari Agricultural University,  Navsari 396450. E. mail:
Abstract: Fig (ficus carica L.) fruits are used for reduce the risk of prostat, breast and colon cancer, helps in reducing blood cholesterol. maintain blood pressure and coronary heart attacks.. They contain health benefiting soluble dietary fiber, minerals, vitamins, and pigment antioxidants. To produce large scale plantation a tissue culture protocol was developed. Maximum establishment of nodal segment explants observed in Murashige and Skoog (MS) medium containing 2.0 mg/l 6- benzylaminopurine (BAP). However, MS medium fortified with 1.0 mg/l BAP + 0.1 mg/l IBA exhibited maximum multiplication rate in second and third sub-cultures. The maximum frequency of multiple shoots in nodal segment explants (83.33 %) was observed on treatment MS + 1.0 mg/l BAP + 0.1 mg/ l IBA. In vitro rooting of regenerated shoot developed in half strength MS medium supplemented with 1.0 mg/l IBA, which produced maximum number of roots per shoot (9) and length of root (3.0). In vitro grown plantlets having 3.0 cm length of shoot were transferred to coco peat media under green house, which showed better survival of plantlets (90.25 %).
Key words: Ficus carica L., Nodal segment explants.
4. Journal of  Cell and Tissue Research  18(2): 6441-6450  (2018)
Recent advances in the development of tropical fish viral vaccines: A review
 Sivasankar, P.,  Riji John, K., Rosalind George, M.,  Magesh Kumar, P., Mohamed Mansoor, M. and Selvamagheswaran, M.
 Department of Fish Pathology and Health Management, Fisheries College and Research Institute,
Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Thoothukudi, Tamil Nadu, India.
 E. mail:,
Abstract: Infectious diseases have led to the most devastating problem in aquaculture sectors. Viral diseases are one of the major challenges to aquaculturists because it is very difficult to control once they occur in the system. Outbreaks of the viral disease lead to a greater economic loss in aquaculture production due to various improper farm managements. Prevention is the only way to control disease incidence caused by viral pathogens. Incorrect use of antibiotics and chemical drugs in aquaculture is associated with several deleterious side effects. Instead of using antibiotics and chemical drugs, vaccination is an effective tool in controlling, preventing, protecting and recovery of fish from virus diseases in cultured fish contributing to sustainability in the aquaculture sector. Therefore, the development of viral vaccines against emergent tropical viral diseases is crucial for promoting successful aquaculture production. The present review was based on different vaccines developed against tropical fish viruses.
Key words: Fish virus, Viral vaccines
5. Journal of  Cell and Tissue Research  18(2): 6451-6456  (2018)
In vitro callus induction and regeneration from leaf explants in Punica granatum l. “Sindhuri
 Kumar, R. and Jakhar, M.L.
Department of Plant Breeding and Genetics , SKN Agriculture University, Jobner
E. mail:,
Abstract: Leaf explant of Sindhuri cultivar of pomegranate were placed on Murashige and Skoog Medium supplemented with different concentration of cytokinins and auxins alone or in combination for callus induction. Maximum callus induction was observed on a medium containing 1.0 mg/l BAP and 1.0 mg/l NAA with 100 per cent frequency followed by 1.5 mg/l BAP with 100 per cent frequency. The highest regeneration of plantlets from callus was obtained upon subculture of calli on the medium containing 1.0 mg/l and 2.0 mg/l NAA. De novo regenerated plantlets induced rooting at 1.0 mg/l IBA and successfully transferred to field after proper hardening.
Key words:  Callus induction, Punica granatum
6. Journal of  Cell and Tissue Research  18(2): 6457-6462 (2018)
Androgenesis-mediated response of different genotypes and their cross combinations for  Calli index in ethiopian mustard (Brassica carinata a. Braun)
Shitole, A.M.and Kumari, V.
R&D Dept., Krishidhan Seeds, D3-D6, Addl. MIDC, Aurangabad road, Jalna, (Maharashtra)-431213.
E. mail:,
Abstract: The effects of seven genotypes and their cross combinations, two basal media i.e., B5 and MS media, two different sucrose concentrations i.e., 3% and 4% sucrose and three combinations of hormones viz. HM1, HM2 and HM3 and their interactions on calli index in Brassica carinata were analyzed by using CPCS software. Mean sum of squares due to all factors were significant revealing thereby significant effects of genotypes, media, hormones, sucrose and their interactions on calli index. Out of all factors and their interactions, the genotype P-51 performed better in B5 medium supplemented with HM2 (0.2mg/l BAP + 2.0 mg/l NAA) and 3% sucrose concentration for high calli index.
                Key words:Brassica carinata ,Calli index
7.Journal of  Cell and Tissue Research  18(2): 6463-6465 (2018)
Electocardiograpic changes in cattle with bovine benign theileriosis
 Devadevi, N., Rajkumar, K. and Vijayalakshmi, P.
Department of Veterinary Medicine, Rajiv Gandhi Institute of Veterinary Education and Research,  Teaching Veterinary Clinical Complex, Pondicherry 605009. E. mail:, 
Abstract: The aim of the study was to assess the electrocardiographic (ECG) changes in cattle with theileriosis of Puducherry region. The study revealed, only bovine benign theileriosis (BBT), which is caused by Theileria orientalis conformed by PCR. In the present study most of the animals suffer from subclinical or chronic form of disease which was indicated by the severe anemic haemogram. The ECG also showed the same picture by significant (P<0.05) increase in the mean QRS amplitude (mV) and Q-T interval (sec) in cattle with theileriosis compared to their respective control mean. Other changes, like sinus tachycardia (62%), sinus bradycardia (12%), Shorten QRS wave (6%), atrial enlargement (9%) and tall T wave (6%) were also recorded.
Key words: Benign bovine Theileriosis, Cattle
8. Journal of  Cell and Tissue Research  18(2): 6467-6471 (2018)
Factor affecting tissue culture experiments of woody plant: A review
 Kumar, R. and  Jakhar, M.L.
Department of Plant Breeding and Genetics , SKN Agriculture University, Jobner
E. mail:,
Abstract: Tissue culture is primarily used for plant propagation and is often referred to as micropropagation. Another way to define the plant tissue culture is the culture of plant cells or plant tissues in a synthetic culture medium under controlled aseptic conditions. The controlled conditions give the culture a suitable micro environment for the successful growth. The tissue culture techniques are successfully used to overcome barriers for plants which are difficult to propagate. Many factors can affect the in vitro establishment as well as micropropagation of different woody plants. Type of explants, physiological status of explant, genotype and age of donor plant, media, plant growth regulators, photoperiod, antioxidants etc. This paper reviews all these aspects related to tissue culture experiment of woody plants.
Key words: Tissue culture, Woody plants
9.Journal of  Cell and Tissue Research  18(2): 6473-6484 (2018)
Effect of heavy metals in physiological alterations and recoveries during natural and herbal antioxidants therapies: A review
 Sood, P.P. and Chundawat, R.S.
Emeritus Medical Scientist, ICMR , New Delhi (Rtd), Emeritus UGC Scientist, New Delhi (Rtd),
 Professor and Head, Department  of Biosciences, Saurshtra University, Rajkot 360 005 (Rtd),
 E. mail:, Cell: 9427270210
Abstract: Considering that none of the therapies used so far has completely recovered the toxic effect of most of the heavy metals, as evident in several biochemical, histopathological or ultrastructural studies, the present review summarizes varies studies dealing with the  combined effect of herbal and inherent antioxidant  therapies used in chromium (VI) and methylmercury intoxications.
Key words:Heavy metals, Herbal antioxidant,  Natural antioxidant
10.     Journal of  Cell and Tissue Research  18(2): 6485-6489 (2018)
Importance of dietary fiber and starch in the prevention of selected civilization diseases: A review 
 Sawicka, B.and Gupta, P.D.
Department of Plant Production Technology and Commodities Science, Faculty of Agrobioengineering,
University of Life Sciences in Lublin, Akademicka 15, 20-950 Lublin, Poland
Abstract:  Starch is a major component of our food; it metabolises easily and gets converted in to sugars which release energy instantly. Diabetic patients are debarred from consuming starchy and sugary foods. World over, potato and rice constitute the major component of human diet; both are starchy foods and forbidden for diabetics. In this review we describe starches from potato and rice can be made useful diet for diabetics.
Key words: Dietary fibers, Starch
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