CTR Publication :: Past Abstracts

1. Journal of Cell and Tissue Research Vol. 23(1): 7263-7266 (2023)

The efficient DNA extraction protocol for single cell microalga (Coccomyxa sp. SUA001)

Kuvad, R.P. And Thaker, V.S.

Plant Biotechnology and Molecular Biology Lab, Department of Biosciences, Saurashtra University, Rajkot, 360 005.
E. mail: thakervs@gmail.com

Abstract: The first step in molecular biology research is to isolate high-quality DNA. The microalga Coccomyxa possess thick cell wall and hence to extract DNA material from those cells is a challenging task. Very limited protocols are available for such single-cell microalga. In this research, we developed a simple, efficient, and high throughput DNA extraction protocol from single-cell Coccomyxa species. The spectrophotometric and gel electrophoresis analyses revealed that the genomic DNA isolated using this approach had a high yield and good quality. The quality of extracted DNA was confirmed using PCR amplification for 18S rRNA gene. This approach is simple and efficient for standard DNA extraction from single-cell microalga.

Keywords: Coccomyxa species, Single-cell microalgae DNA extraction

2. Journal of Cell and Tissue Research Vol. 23(1): 7267-7272 (2023)

Light, sleep and obesity- Is there any connection between them?

Gupta, P.D. and Sood, P.P.

Former Director Grade Scientist, Centre for Cellular and Molecular Biology, Hyderabad,

E. mail: pdg2000@hotmail.com

Abstract: Before humans evolved on this planet Nature created conditions for him to remain healthy and active. Among those, sleep is one. To sleep better, dark and light cycles were created by the rotation of the earth. However, man has modified the condidions by creating artificial light which reduced the dark hours and due to this many health problem are produced. Researchers started studying these problems and found many direct and indirect links for the deteriorating health issues; recent experimental and epidemiological data indicated that exposure to nightlight is responsible for diseases such as cancer, obesity and depression. These studies caution us for the judicious use of light. Here we have put forth reasons for increasing obesity global.

Keywords: Light, Dark, Sleep, Obesity interaction

3. Journal of Cell and Tissue Research Vol. 23(1): 7273-7277 (2023)

Solanum surattense CP genome sequencing, its annotation and comparative genomics with allied species of genus Solanum

Nandaniya, N.S. Bhatt, P.P. Chudasama, K.S. And Thaker, V.S.

Plant Physiology and Molecular Biology Lab. Department of Biosciences, Saurashtra University, Rajkot 360005, Gujarat, India, Vimal research Society for Agribiotech, Agi Road, Rajkot, 360 003, Gujarat, India.

E mail: thakervs@gmail.com

Abstract: Solanum surattense is also called as Yellow Berried Nightshade, is a member of Solanaceae family. It grows as a weed along roadsides and in waste lands throughout India. S. surattense is a traditional medicinal plant having numerous medicinal properties. In present study cp DNA is extracted from S. surattense and it is subjected to high throughput sequencing. Raw reads were tested for quality and any leftover adapter contamination removed using FastQC v0.11.5. The clean raw cp genome was assembled using CLC Genomic workbench v9.5.64. The assembly was performed in the reference-assisted mode using the reference sequence. From the complete CLC assembly results, assembled contigs with more than 50X coverage compared to complete cp genome of Solanum anomalostemon (NC_062874.1) as the reference genome.Annotation of complete chloroplast genome was performed using online tool CpGAVAS2. The circular map of cp genome was accomplished by CpGAVAS2 and OGDRAW. Geneious was used for measurements of gene size, regions comparison and GC content calculation from the cp genomes. The size of chloroplast genome of S. surattense was 154497 bp with overall 37.70% GC content. It exhibited quadripartite structure with 85957 bp of LSC region, 20516 bp of SSC region and 24012 bp of each IR region. A total 131 genes were identified, including 85 coding genes, 38 tRNA genes and 8 rRNA genes. It was compared with Solanum anomalostemon cp genomes obtained from the NCBI database and differences in the genome size, GC content, number of genes and tRNA numbers, were observed.These data can be explore for identification and authentication of the plant.

Keywords: Solanum surattense, Chloroplast genome, Solanaceae

4. Journal of Cell and Tissue Research Vol. 23(1): 7279-7288 (2023)

Tissue culture techniques in pomegranate micropropagation: A review

Ravi Kumar

School of Agriculture, RNB Global University, Bikaner. E. mail: ravi.bishnoi@rnbglobal.edu.in,

Abstract: Micropropagation is a vegetative propagation under fully controlled condition, which required aseptic conditions in the microenvironment of the culture vessel. These culture vessels have the all-growth requirements of a plant in the natural conditions. Scientists developed different techniques of propagation in last few decades. These techniques could facilitate large scale production of plants which helps for the improvement of the different species. Recently pomegranate micropropagation techniques are applied in order to produce large numbers of new high-quality plants in a relatively short time and space, in low cost and can also be preserved. An overview on the in vitro propagation via meristem culture, callus culture and nodal segment culture etc. are presented here.

Keywords:-Micropropagation, Pomegranate