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Tissue Research » Past Abstracts
1. Journal of  Cell and Tissue Research  17(1) 5937-5943 (2017)
The possible protective role of the pomegranates juice on the testis of aged male albino rats
Yassien,  R. I.
Department of Histology, Faculty of Medicine, Menoufia University. 6 Abdel-Rehim Badawy St. from Dr. Mostafa El-Naggar St., ShebinElkom - 32511, Menoufia, Egypt.  E. mail:
Abstract: This study was carried out to investigate effect of Pomegranates juice (PJ) on the testis of aged male albino rat. PJ is a strong antioxidant used for many medical disorders. Animals were randomly divided into 3 groups of ten rats for control group and fifteen rats for each other two groups. In (group I) and (group II) animals were received distilled water by gavage daily for 52 days. Where in (group III) animals were received PJ by gavage at a dose of 10 ml/kg/day daily for 52 days. All animals were sacrificed at the end and testis were separated and processed. Light microscopic studies of aged animals demonstrated loss and degeneration of germ cells, sertoli and leyding cells. Excessive collagen deposition in aged group was detected. Morphometric studies exhibited decreased epithelium height, diameter of STs in aged group. While biochemical studies showed decreased testosterone level and increased FSH& LH. PJ group showed improved pictures nearly similar to control group. Therefore, it is noticed the beneficial effect of pomegranate juice on testis in aged rats that may makes it one of the most important foods for the future.
Key words: Pomegranates juice, Aging, Rats 

2. Journal of  Cell and Tissue Research 17(1) 5945-5960 (2017)
Comparative study of protective effect of olive oil with or without gentamicin on E. coli-induced lung sepsis in  adult male albino rat.
Aboraya, A. and Ali, A.M.
Department of Histology, Faculty of Medicine, Tanta University, Egypt; 2Department  of Medical Physiology, Faculty of Medicine, Fayoum University, Egypt. E. mail:
Abstract: Sepsis is frequently the cause of severe pulmonary dysfunction. Gentamicin is used clinically due to its wide spectrum of activities against infections; however it has serious adverse effects and life-threatening toxicity. The challenge for scientists is to develop alternative therapy or protective agents against its toxicity. Hence the present study is an attempt to elucidate the possible protective role of virgin olive oil with or without gentamicin against the toxic changes on the lung induced by sepsis. Thirty six adult male albino rats were used and divided into three groups. Group I: served as the control group, Group II: lung sepsis was induced by intraperitoneal injection of E. coli LPS serotype O127 : B8 and Group III: lung sepsis was induced as in group II then divided randomly into 3 subgroups. Subgroup IIIa: animals were injected with gentamicin, Subgroup IIIb: animals were injected with gentamicin and given virgin olive oil and Subgroup IIIc: animals were given virgin olive oil only. During the experiment the body weight and hemodynamic parameters were monitored. At the end of the experiment the lungs were excised out and processed. Histological and immunohistochemical studies of lung sepsis showed an alteration in the lung architecture in the form of overexpansion of alveoli alternating with collapse of others. Pneumocytes II showed destruction of lamellar bodies and were predominant replacing pneumocytes type I in the alveolar lining with increase in HSP70 expression, significant decrease in body weight and blood pressure with tachycardia. Gentamicin- treated group revealed minimal improvement in lung tissue. While concomitant administration of virgin olive oil along with gentamicin showed a noticeable improvement in lung architecture and increase in HSP70 expression with significant improvement of body weight and blood pressure. However animals given virgin olive oil only revealed the most favorable results with remarkable improvement of lung architecture. In conclusion, based on the previous findings, gentamicin and olive oil prevents the deleterious effects of lung sepsis. Moreover, virgin olive oil solely has succeeded to improve the septic effects on lung by enhancing anti-oxidant defense system and suppression of oxidative stress.
Key words: Olive oil, Gentamicin, Lung sepsis, HSP70
3. Journal of  Cell and Tissue Research 17(1) 5961-5972 (2017)
The possible protective role of curcumin on nicotine induced damage of gastric mucosa of adult male albino rats
Aboraya, A.
Department of Histology; Faculty of Medicine; Tanta University. Al-Garbia, Egypt. E. mail:
Abstract: Nicotine has been reported to cause deleterious changes on gastric mucosa such as gastritis, gastric ulcer and even has been well documented to be closely related to the development of gastric cancer. This study was carried out to evaluate the possible gastroprotective effect of curcumin along with its effect on cyclooxygenases against cytotoxicity of nicotine in gastric mucosa. Forty five adult male albino rats were randomly divided into three groups of fifteen animals in each group; group I (Control), group II: each rat was given oral nicotine (50 μg/ml) once daily, group III: each rat was given oral curcumin (200mg/kg) body weight once daily concomitant with nicotine with similar dose as group II. All animals were treated for 21 days and were sacrificed at the end. The stomach was processed for histological and immunohistochemical studies. Nicotine administration induced erosion and disruption of fundic gastric mucosa with exfoliated remnant of gastric glands associated with dilated, congested blood vessels and apical new vascularization. Marked decrease in PAS positive mucus with highly significant decrease in COX-1 expression and a highly significant increase in COX-2 expression were observed. Concomitant use of curcumin with nicotine showed considerable degree of preservation of gastric mucosa and PAS positive mucus. Anon significant decrease in COX-1 expression as well as a significant increase in COX-2 expression were observed. In conclusion, curcumin showed a protective role against the harmful effects of nicotine on the gastric mucosa.
Key words: Nicotine, Gastric mucosa, Curcumin, Cyclooxygena 

4. Journal of  Cell and Tissue Research 17(1) 5973-5982 (2017)
Low dose heterogeneous chemical mixture exposure induced alterations in the structure and antioxidant defense system of rat liver
Vachhrajani, K.D.  and Morya, K.
Division of Environment and Toxicology, Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara-390002m  Gujarat. E. mail:
Abstract: Adult male rats were exposed to heterogeneous chemical mixture (HCM) of Phthalic acid di butyl ester, 1, 2 –Dichlorobenzene, Cadmium chloride and Chromium trioxide, through oral gavage, at a dose level equal to 1/100 and 1/1000 of LD50 value of each chemical compound for 60 days followed by withdrawal of exposure until 120 day of experiment. Histological alterations, serum parameters of liver function and oxidative stress parameters in liver assessed the status of structure and function of liver following exposure. The increased serum liver marker enzyme activities and decreased endogenous antioxidant levels suggested that HCM exposure below individual chemical’s NOAEL doses significantly hamper the normal function of liver. This is well correlated with increased CHO, LDL and VLDL level because these appear harmless until they are in blood vessel walls and oxidised by free radicals. Thus, HCM exposure has a definite effect on liver structure and function. However, the low dose HCM induced changes are reversible and effectively reversed by withdrawal of treatment for comparatively longer duration of time.
Key words: Industrial chemical mixtures, Liver
5. Journal of  Cell and Tissue Research 17(1) 5983-5986 (2017)
Comparative study of certain serum biochemical and mineral profile in postpartum anestrus cows treated with CIDR and Ovsynch protocol
Jena, D., Das, S., Samal, P., Behera, S. and Mohanty, D.N.
Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, 243122, Bareilly (UP). 
E. mail:
Abstract: Thirty postpartum anestrus jersey crossbred cows were selected and divided into 3 groups with equal number of animals. The group 1 animals were administered with an intravaginal progesterone device (CIDR) on day 0 and 2 ml of Prostaglandin F2α on 7th day i.e., on day of CIDR removal whereas group 2 cows were treated with ovsynch protocol (GnRH - PGF2α- GnRH) on day 0, 7 and 9 respectively. The group 3 cows were supplemented with mineral mixture 100 gm daily for 15 days and treated as control. The animals were inseminated by frozen semen at a fixed time, 48 h after PGF2α injection in group 1 and 24 h after second GnRH injection in group 2. However, the group 3 animals were served by frozen semen at observed estrus. The study was conducted with an objective to determine the serum mineral concentration (serum calcium, serum phosphorus and their ratio) and cholesterol level. The serum cholesterol was estimated by CHOD/PAP method whereas the serum phosphorus concentration was estimated by molybdate U.V method and serum calcium concentration was done by OCPC method by using Coral kit. The absorbance of standard and test sample was measured against the blank by semi auto analyser at wavelength of 505nm, 340 nm and 570nm. There was no significant difference for serum calcium, phosphorus, cholesterol in postpartum anestrus cows irrespective of treatment protocols and days of sampling.
Key words: Jersey crossbred cow, CIDR, Ovsynch,

6. Journal of  Cell and Tissue Research 17(1) 5987-5990 (2017)
 Initiation of callus culture and estimation of andrographolide from Andrographis paniculata
Deshmukh, A.S., Kadam, S.R., Belge, S.A., Nirgude, M.S. and Kharade, S.S.
K. K. Wagh College of Agricultural Biotechnology, Nashik, Maharashtra 422003.
E. mail:
Abstract: Andrographis paniculata is an herbaceous plant found commonly in India and Sri Lanka. It has high medicinal use in several diseases like diarrhea, cholera, diabetes, influenza etc. A rapid and efficient in vitro method has been standardized for initiation of callus culture from leaf disc explants obtained from 45-day old aseptic seedling. Seeds were germinated on MS media. The germination percentage was 99% after 45 days. For callus initiation 45 days old sterilized leaves were transferred in MS medium containing different concentration of hormones (2,4-D 0.5, 1, 1.5, 2, 2.5 and 3 mg/L and 2,4-D 1mg + Kinetin 1mg/L) and 100% callus induction was observed in all concentrations of hormones. Compact and green callus was formed in 2,4-D + Kinetin at 1mg/L concentration. Ethanol extract of callus was used for quantitative estimation of andrographolide. HPLC analysis showed that retention time of standard andrographolide was 2.7 minutes and that of sample was 2.66 minutes. Study shows that andrographolide concentration in sample was about 0.87mg/L.
Key words: Andrographis paniculata,Andrographolide 

7. Journal of  Cell and Tissue Research 17(1) 5991-5996 (2017)
Seroprevalence of canine leptospirosis in Namakkal, Tamil Nadu by microscopic agglutination test
Sathiyamoorthy, A., Selvaraju, G., Palanivel, K.M. and Srinivasan, P.
Department of  Veterinary Preventive Medicine, Veterinary College and Research Institute, Namakkal - 637 002 (Tamil  Nadu). E. mail:
Abstract: Leptospirosis is a re-emerging and a complex zoonotic bacterial disease, caused by pathogenic serovars of Leptospira interrogans. 250 sera samples from clinically affected and apparently healthy dogs were collected along with relevant epidemiological data at Teaching Veterinary Clinical Complex, Veterinary College and Research Institute, Namakkal for a period of one year from May 2014 to April 2015 and subjected to Microscopic Agglutination Test (MAT). Overall prevalence of canine leptospirosis was found to be 28.40%. Serovar prevalence indicated that grippotyphosa was the predominant serovar for about 35.21% followed by 28.62% of autumnalis, 22.53% of australis, 16.90% of pomona, 15.49% of canicola, 12.67% of icterohaemorrhagiae, 12.67% of pyrogenes and 1.40% of tarassovi. Out of 71 MAT positive serum samples, 18 (25.35%) were cross-reacted with more than one serovars. Risk factors were assessed by Chi-square test and found that age, sex health status and working nature were significantly associated with the occurrence of leptospirosis in dogs.
Key words: Canine leptospirosis, Leptospira interrogans

8. Journal of  Cell and Tissue Research 17(1) 5997-6001 (2017)
Hormonal, antioxidant and enzymatic profiling in cross-bred cattle (Bos tauraus)
Chetia, M., Sarma, S., Tamuly, S., Nath, R., Goswami, J., Mili, D.C. and Das, P.K.
College of Veterinary Science, Khanapara, Assam Agricultural University, Guwahati 781022 (Assam).
E. mail:,
Abstract: The present experiment was undertaken to study the effect of summer and winter season on the hormone and enzymatic profile of cross-bred (HF X J) cattle in Assam. The different hormones such as Cortisol, T3 and T4 are estimated by RIA technique and the different enzyme activities such as SOD (Superoxide Dismutase), GSH-Px (Glutathione Peroxidase) and CAT (Catalase) are analysed by different standard methods while LDH (Lactate Dehydrogenase) activity was estimated by using commercially available kit. It has been observed that all the stress related hormones and the erythrocytic enzyme activities were increased in summer compared to winter season and the difference was highly significant (P<0.01). The increase in hormone concentration and enzyme activity might be attributed to the thermal stress in the summer.
Key words: Biochemical profile, Cross-bred cattle, Seasonal changes

9. Journal of  Cell and Tissue Research 17(1) 6003-6010 (2017)
KI 67 proliferation antigen in spontaneous canine cutaneous and subcutaneous tumours and its prognostic importance
Kavya, N., Rao, S.,  Sathyanarayana, M.L.,  Narayanaswamy, H.D.,  Byregowda, S.M.,  Ranganath, L.,  Kamaran, A.,  Purushotham, K.M. and Kishore, T.K.
Department of Veterinary Pathology, Karnataka Veterinary Animal and Fisheries Sciences University [KVAFSU], Veterinary College, Hebbal, Karnataka. E. Mail:,
Abstract: Purpose of the study was to evaluate the prognostic value of Ki 67 proliferation antigen in spontaneous canine cutaneous and subcutaneous tumours by immunohistochemistry. Formalin fixed, paraffin embedded histological sections were immunostained with monoclonal anti-human Ki 67 antibody (GM001). To determine Ki 67 index, approximately 1000 neoplastic cells were counted in 10 representative fields and number of positive cells per 1000 cells was expressed as percentage. Malignant tumours (64) had a mean Ki 67 index of 24.65 ± 1.69 while benign tumours had an index of 11.38 ± 2.61. Statistically significant difference (P < 0.05) was observed between malignant and benign types. The mean Ki 67 expression among the 12 dogs with malignant tumours that died was significantly (P < 0.05) higher (37.37 ± 4.32 per cent) compared to alive group. In 16 dogs with recurrence also the Ki 67 index was significantly (P < 0.05) higher (29.06 ± 2.74 per cent) as compared to alive group. The results of the current study indicated that poor prognosis was associated with higher Ki 67 index and the survival analysis, using Ki 67 index median cut off value indicated the prognostic importance of Ki 67 index in malignant cutaneous and subcutaneous tumours of dogs.
Key words: Cutaneous - subcutaneous tumours, Ki 67 antigen,

10.  Journal of  Cell and Tissue Research 17(1) 6011-6014 (2017)
Hormonal profile of estrous synchronized Black Bengal does by the use of different progesterone therapies
Dash, S., Mohanty, D.N., Jena, B. and Mandal, K.D.
Department of Animal Reproduction, Gynaecology and Obstetrics,  College of Veterinary Science and Animal Husbandry, OUAT, Bhubaneswar-751003, (Odisha). E. mail:
Abstract: A total of 60 normal healthy breedable Black Bengal does from first to third parity were taken for present study. These animals maintained on concentrate and foraging were divided into three different groups. Group I (n=20) animals were synchronized for oestrous with intravaginal sponges (AVIKESIL-S) impregnated with natural progesterone and the sponges were left in situ for 15 days. Group II (n=20) goats received long acting progesterone (p-depot) through intramuscular route at dose rate of 50 mg (0.2 ml/doe) at an interval of 3 days for 5 occasions. Both the group of does received 125 microgram of cloprostenol on the day of sponge withdrawal and after completion of intramuscular progesterone treatment, respectively. Group III (n=20) animals maintained under routine managemental practices receiving no such treatment were served as control. Intravaginal sponge impregnated with progesterone (P4) induced substantial increase of serum P4 after completion of therapy. A high concentration of estrogen and low progesterone concentration was consistently observed in the day of estrous.
Key words: Gonadal hormone, Intravaginal sponges, Goats

11. Journal of  Cell and Tissue Research 17(1) 6015-6019 (2017)
Effect of growth regulator concentrations on callus induction and regeneration in japonica rice varieties through anther culture
Sharma, A., Lal, D., Sutradhar, M., Pradhan, H. and Kumari, N.
Department of Plant Biotechnology, University of Agricultural Sciences, GKVK, Bengaluru.
E. mail:
Abstract: Rice (Oryza sativa L.) is one of the most important food crops of Southeast Asia, which feeds half of the world population. Anther culture is an efficient and convenient technique for rapid production of doubled haploids which are useful in crop breeding programs. The combination of different growth regulators facilitates callus induction on N6 medium and regeneration on MS medium. Highest callus induction frequency was recorded in growth regulator concentrations of 2, 4-D 2.0 mg/l+ Kinetin 1.0 mg/l (34.54%) followed by in growth regulator concentrations 2, 4-D 1.0 mg/l + NAA 2.0 mg/l + Kinetin 0.5 mg/l (33.38%). Highest regeneration frequency was recorded in growth regulator concentrations of Kinetin 0.5 mg/l + BAP 2.0 mg/l + NAA 1.0 mg/l (69.44%) followed by in growth regulator concentrations of Kinetin 2.0 mg/l + BAP 1.0 mg/l + NAA 1.0 mg/l (40.00%). This is clearly indicated that type of growth regulator along with its concentration plays important role in callus induction and regeneration in japonica rice varieties.
Key words:Oryza sativa L., Anther culture, Growth regulators

12.Journal of  Cell and Tissue Research 17(1) 6021-6034 (2017)
The effect of bisphenol a on the testis of adult male albino rats and the possible protective effect of thymoquinone: A histological and immunohistochemical study
Fahmy,  A.
Histology Department, Faculty of Medicine, Menoufia University, 5 Ibrahim El-Tory st. Quweisna, Menoufia 048, Egypt.
E. mail:
 Abstract: Bisphenol A (BPA) is an endocrine-disrupting molecule and can bind to α and β estrogen receptors and exerts its harmful effects. It is widely used in epoxy resins and polycarbonate plastics. Exposure to BPA can be associated with many health problems as abnormalities of reproductive system, cardiovascular disease, metabolic disorders, cancer and neurobehavioral disorders. Thymoquinone (TQ) have antioxidant, anticonvulsant, antitumor and anti-inflammatory effects, and may protect organs from oxidative damage by free radical generating agent. The objective of this study is to evaluate the protective effect of thymoquinone against BPA induced germ cell toxicity of adult male albino rats testis. In this study, forty adult male rats were used. They were divided into four groups (10 rats for each): group I (control group), group II (thymoquinone treated group), group III (bisphenol A treated group), group IV (bisphenol A and thymoquinone treated group). Testicular tissue were used for histological and immunohistochemical studies. Blood plasma was used for hormonal analysis. Administration of BPA significantly reduced seminiferous tubules diameter and epithelial height with impaired spermatogenesis, decreased plasma testosterone, FSH and LH levels. BPA caused decreased androgen receptors. Administration of thymoquinone with bisphenol A led to amelioration of these toxic effects. Thus exposure to BPA induces toxic effects on the testis with an imbalance in the hormonal levels, while thymoquinone could ameliorate these toxic effects.
Key words: Rat testis, Bisphenol A, Thymoquinone

13.Journal of  Cell and Tissue Research 17(1) 6035-6041 (2017)
Phytochemical and GC-MS studies on traditional herbaceous plant pumpkin (Cucurbita pepo) leaf
Arul Priya, R. and Saravanan, K.
Post Graduate and Research Department of Zoology, Nehru Memorial College (Autonomous), Puthanampatti 621 007, Tiruchirappalli, Southern India. E. mail:
Abstract: To analyze the presence of phytochemical compounds from the leaves of Cucurbita pepo (Pumpkin) by preliminary phytochemical screening and GC-MS studies. The dried powder leaves of C. pepo were extracted successfully by Soxhlet using ethylacetate and hexane solvents. All the prepared extracts were used to preliminary phytochemical screening. GC-MS analysis was performed to identify the phytocompounds present in the ethylacetate and hexane extracts of C. pepo leaves. Phytochemical analysis of extracts showed the presence of major classes of phytochemicals. GC-MS results revealed the presence of 7 phytoconstituents in ethylacetate extract and 4 phytoconstituents in hexane extract. Among these, α-amyrin, phytol and morin are medically important. Results of this study may provide a foundation for designing new drug for several diseases.
Key words: Phytochemical screening, GC-MS analysis, Cucurbita pepo.

14.Journal of  Cell and Tissue Research 17(1) 6043-6046 (2017)
 Genetic variation in selected species of prawns of genus macrobrachium using mitochondrial 16S rRNA
Sharma, C., Krishna, G., Pavan Kumar, A. and Nayak, S.K.
Mangalore University Mangalagangotri 574199 (Karnataka).  E. mail:
Abstract: Mitochondrial partial 16s ribosomal gene was used to infer the genetic variation of species of genus Macrobrachium. From 481 bases, the number of variable sites for Macrobrachium rosenbergii is 236, Macrobrachium villosimanus is 34, Macrobrachium lar is 119 and Macrobrachium idella is 105. The average nucleotide frequencies were A: 35.1%; T: 29.8%; C: 22.4%, G: 12.6% and the A+T rich sequences was 64.9%. The Ts/Tv ratio is 27. More number of haplotypes was seen in Macrobrachium rosenbergii population. Neutrality index Tajima’s D value of Macrobrachium rosenbergii population was negative. High haplotype and low to moderate nucleotide diversity were seen for all the populations of different species and the populations are expanding in size.
Key words: Genetic variation, Macrobrachium, 16s rRNA

15.Journal of  Cell and Tissue Research 17(1) 6047-6050 (2017)
 Evaluation of the cytotoxic activity of crude aqueous extracts of some medicinal plants  of  Kashmir
 Reshi, I.A., Sarkar, T.K., Malik, H.U., Muhee, A. and Bhat, A.H.
 Department of Clinical Veterinary Medicine, Ethics and Jurisprudence, Faculty of Veterinary and Animal
Science, SKUAST-K  (J&K).  E. mail:,
Abstract:  The objective of this study was to evaluate the cytotoxicity potential of aqueous extracts of Nepeta cataria, Fumaria indica, Borago officinalis, Adiantum capillus, Levandula stoeches on HeLa cell line. Leaves of plants were washed with distilled water, dried in shade, grinded to fine powder and stored in airtight container at room temperature in the dark until used. The powdered samples were subjected to extraction using distilled water. Different concentrations of the extracts of herbals were prepared. Cytotoxicity study was carried out by The MTT cell proliferation assay on HeLa cell line which measures the cell proliferation rate and conversely, when metabolic events lead to apoptosis or necrosis, there is reduction in cell viability. Fumaria indica exhibited cell viability ranging from 89.60 to 100 per cent against the extract concentration of 5 to 100μg/ml with IC50 (µg/ml) on HeLA cell line as -581.79 Adiantum capillus extract revealed cell viability from 81.91 to 100 per cent against the extract concentration of 5 to 100 μg/ml with IC50 (µg/ml) on HeLA cell line as 36.96. Nepata cataria extract showed cell viability ranging from 84.36 to 96.09 per cent against the extract concentration of 5 to 100 μg/ml with IC50 (µg/ml) on HeLA cell line as -112.07. In case of  Levandula stoeches extract per cent cell toxicity ranged from 0 to 41.77 against the extract concentration of 5 to100μg/ml. Borago officinalis extract exhibited per cent cell toxicity ranging from 4.27 to 43.17 against the extract concentration of 5 to100 μg/ml with IC50 (µg/ml) on HeLA cell line as 26.91.
Key words: Medicinal plants, Cytotoxicity

16. Journal of  Cell and Tissue Research 17(1) 6051-6054 (2017)
Pinnal squamous cell carcinoma in a cat:  A case report
 Naveen, M., Dhanalakshmi, S. and Kavitha Rani, B.

Department of Veterinary Surgery and Radiology, Veterinary College, Vinobanagara,Shivamogga 577204, Karnataka.
E. mail:

Abstract: A white male cat aged 2.5 years was presented to Teaching Veterinary Clinical Complex, Veterinary College, Shivamogga with a history of abnormal growth on both the ears since 1 month and cat was taking food and water normally. Clinical examination revealed presence of ulcerated tumor like growths on the right ear pinna and dermatitic lesions on the left ear pinna. The condition was tentatively diagnosed as squamous cell carcinoma of ear pinna and it was decided to perform partial pinnectomy. Surgical site was prepared asceptically. Anaesthesia was performed using Inj Calmpose 3mg IM and Inj Ketamine 1.6ml IM. Partial pinnectomy was performed in the right ear to remove portion of the ear pinna affected with SCC and in the left ear to remove the portion of ear pinna affected with dermatitis. Sample was collected for histopathology. Cat was kept on Susp Cefpet for another 7 days. Cat recovered without any complications. Histopathological findings confirmed the condition as squamous cell carcinoma of ear pinna.
Key words: Squamous cell carcinoma, Ear, Cat 

 17.Journal of  Cell and Tissue Research 17(1) 6055-6061 (2017)

Multiplex PCR assay for detecting common bacterial pathogens of mastitis in milk and tissue samples of buffaloes
Sarvesha, K., Satyanarayana, M.L., Narayanaswamy, H.D., Rao, S., Yathiraj, S., Isloor, S.,  Srikanth, M.and
Mukartal, S.Y.
Department of Veterinary Pathology, Veterinary College, Hebbal, KVAFSU, Bangaluru 560 024. 
E. mail:
Abstract: The present study was undertaken to identify the seven common bacterial pathogens of  mastitis viz., Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Staphylococcus chromogenes (S. chromogenes), Streptococcus agalactiae (S. agalactiae), Streptococcus dysgalactiae (S. dysgalactiae), Streptococcus uberis (S. uberis) and Escherichia coli (E. coli) simultaneously in the milk and tissue samples of mastitis affected buffaloes collected from Kolar and Chikkaballapur districts, Karnataka, India by Multiplex PCR (m-PCR) based microorganism detection technique. A total of 65 milk (50 SCM, 15 CM) and 61 tissue (36 SCM, 25 CM) samples were collected  from mastitis affected buffaloes were used to identify seven common bacterial pathogens by m-PCR. Milk samples were screened for subclinical mastitis (SCM) by California mastitis test (CMT). Based on m-PCR results, S. aureus was found to be the predominant bacteria detected from milk and tissue samples followed by E. coli, S. agalactiae, S. epidermidis, S. chromogenes, S. dysgalactiae and  S. uberis. Eight milk and seven tissue samples were negative for seven targeted bacterial species and also 10 milk and 9 tissues were positive for mixed infection consisting of more than one bacterial species. Present study supported the fact that m- PCR based identification of mastitis causing pathogens from milk is a rapid and reliable method to reveal the exact bacterial etiology of mastitis.
Key words: Subclinical mastitis, Bacterial pathogens, Buffaloes milk
18.Journal of  Cell and Tissue Research 17(1) 6063-6065 (2017)
Nanopore technology for DNA sequencing aids in clinical diagnosis of genetic disorders in newborn screening:
A mini review
Gupta, P. D.
Manipal University, Manipal, Karnataka, India
Abstract: Since its inception many modifications have been taken place and now nanopore technology has become technology of choice for DNA sequencing. Since it is simple, quick, accurate and inexpensive, it is very useful for detection of inborn metabolic disorders; sometimes patients of such diseases do not survive for long and clinician needs quick results. Moreover this technique dose not required large sample size.
Key words: Nanopore, DNA sequencing, Genetic disorders
19.Journal of  Cell and Tissue Research 17(1) 6067-6072  (2017)
Pathomorphological studies on ovine paratuberculosis in an organised sheep farm in Karnataka
Srikanth, M., Narayanaswamy, H.D., Satyanarayana, M L., Suguna Rao., Rathnamma, D., Ranganath, L., Mukurtal,  S.Y., Sarvesha, K.  and Manjunatha, S.S.
Department of  Veterinary Pathology Veterinary College, Hebbal, Bengaluru 560024.  E. Mail:
Abstract: Paratuberculosis (Johne’s disease) is one of the major economically important disease of small ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP) – a slow growing acid fast bacterium. It causes granulomatous enterocolitis, lymphadenitis and lymphangitis in domestic and wild ruminants. The present study was conducted to record pathomorphological changes in naturally occurring ovine paratuberculosis in an organised sheep farm. The study included 36 paratuberculosis suspected morbid cases in an organised Bannur sheep farm in Mandya district, Karnataka, India. Clinical findings such as intermittent diarrhoea, jowl oedema, cachexia, anaemia, paleness of mucous membrane were recorded in paratuberculosis suspected cases. Out of 36 paratuberculosis suspected morbid cases, 12 showed gross lesions such as muscular atrophy, thickening and corrugation of intestines especially at ileocaecal junction, cording of mesenteric lymphatics with enlarged and oedematous lymph nodes. Gelatinous fat atrophy was very conspicuous in omental, mesenteric, pericardial and perirenal fat. Microscopically lesions were classified on the basis of their location in relation to intestinal lymphoid tissue, intensity, inflammatory cell types and numbers of mycobacteria. In four sheep, paucibacillary type of lesions were observed which were characterized by small granulomas in villi, lamina propria and lymphoid follicles. The granulomas contained few macrophages with clear and large nuclei and abundant cytoplasm with heavy infiltration of lymphocytes. Eight cases revealed mulitbacillary type of lesions with large granulomas characterized by extensive infiltration of macrophages and epitheloid cells along with acid fast organisms with few lymphocytes infiltration. In conclusion, ovine paratuberculosis was diagnosed  based on classical gross and histopathological lesions were confirmed positive by IS900 specific PCR in an organised semi intensive sheep farm in Karnataka in Bannur sheep breed.
Key words: Paratuberculosis, Multibacillary, Paucibacillary, Bannur sheep 

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