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1. Electronic Journal of Pharmacology & Therapy 6: 1-5 (2013)
ISOLATION AND CHARACTERIZATION OF BIOACTIVE STREPTOMYCES FROM AGRICULTURAL SOIL
Subash, N. and Sasikumar, C.
PG & Research Department of Biotechnology, Nehru memorial College (Autonomous), Affiliated
to Bharathidasan University, puthanampatti 621 007, Tiruchirappalli, Tamilnadu.
E. mail: biosubash777@gmail.com.
Abstract: Actinomycetes have long been recognized as the prime sources of antibiotics, enzymes and of other important metabolites. The soil samples were collected from the agricultural land Thakkolam, Vellore district and isolation of actinomycetes and screening for antibacterial activity using Agar Plug Method against E. coil, Pseudomonas, Klebsiella, Staphylococcus, Streptococcus, and Bacillus species was carried out. Based on the results one isolate Streptomyces sp. AS-9 was observed to be more potent than other isolates and selected for further studies. The bioactive substance from the broth of Streptomyces sp. AS-9 was extracted with different solvents like ethyl acetate and methanol and the crude compound was concentrated by evaporation. The crude extract was further purified using TLC and used for Secondary screening for antibacterial activity using well and disc diffusion methods. The results of the antibacterial activity of selected isolate Streptomyces sp. AS-9 against test bacterial cultures E. coil, Klebsiella, Pseudomonas, Staphylococcus, Bacillus and Streptococcus was determined by agar well diffusion method. Streptomyces sp. AS-9 produced maximum zone of inhibition against Pseudomonas sp. (15 mm) and minimum against E. coli (9 mm). The antibacterial activity of Streptomyces sp. AS-9 against test bacterial cultures E. coil, Klebsiella, Pseudomonas, Staphylococcus, Bacillus and Streptococcus was determined also by disc diffusion method.
Key words: Streptomyces, bioactive compounds, antimicrobial activity
2. Electronic Journal of Pharmacology & Therapy 6: 7-11 (2013)
PROPHYLACTIC ROLE OF A POLY HERBAL FORMULATION ON ALLOXAN INDUCED DIABETES IN EXPERIMENTAL MODELS
Radhika, J., Jothi, G. and Ahaliya, K R.
Srimad Andavan Arts and Science College, Tiruchirappalli 620005; E. mail: radiarun2005@yahoo.co.in
Abstract: Diabetes is an epidemic disorder, including chronic complications characterized by damage, dysfunction and eventual failure of various organs, especially the eyes, kidneys, nerves, heart and blood vessels. The rapidly increasing incidence of diabetes mellitus is becoming a serious threat to mankind health in all parts of the world. The traditional medicine proved to possess good therapeutic value showing a bright future in the treatment of diabetes mellitus. The present study was designed to evaluate the antidiabetic potential of a poly herbal formulation comprising of Biophytum sensitivum Linn., Bambusa arundinacea Retz., Artocarpus heterophyllus Lam., Trigonella foenum-graecum Linn. Albino rats of either sex were used as experimental models. The rats were divided into six groups each comprising of six rats each. The groups were group I- normal control, group II- alloxan induced disease control (150mg/kg b.wt), group III- alloxan+ formulation (500mg/kg b.wt), group IV- alloxan + formulation (750mg/kg b.wt), group V- alloxan + glibenclamide (200mg/kg b.wt) and group VI- formulation treated (750mg/kg b.wt) respectively. After the experimental period of 45 days, the blood and tissues were collected and pre-clinical trials were carried out. The parameters studied were plasma glucose, hepatic glycogen, serum insulin, glycosylated hemoglobin, glucose-6-phosphatase, glucokinase, and serum marker enzymes (AST, ALT and ALP). Alloxan induced disease control group showed significant increase in plasma glucose, glycosylated hemoglobin, glucose-6-phosphatase, and serum marker enzymes viz., AST, ALT and ALP. It also showed significant decrease in hepatic glycogen, glucokinase, and serum insulinlevels. Oral administration of the formulation restored the level of biochemical parameters and serum marker enzyme levels. From the present observation, it is evident that the formulation could be an effective antidiabetic drug without any toxicity.
Key words: Poly herbal formulation, Alloxan, Diabetes
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