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1. Journal of Cell and Tissue Research Vol. 23(2): 7289-7294 (2023)
Diagnostic reliability of thyroid imaging reporting and data system (TIRADS), a non invasive classification in differentiation between benign and malignant thyroid lesions
Thejashwini, S.I., Shetty Prasad, S., Suresh Badiger, S., Udupa, N. and Srinivas Pai, B.
Department of Surgery Sri Dharamsathala Manjunatheshwra College of Medical Sciences and Hospital Sri Dharamsathala Manjunatheshwara University, Sattur, Dharwad, Karnataka. E. mail: srinpai@gmail.com,
Abstract: Prevalence of thyroid nodules is increasing all over the world. An ultrasound based thyroid imaging reporting and data system classification has been developed . To classify thyroid nodules and address the issue of nodule selection for FNAC, a useful thyroid imaging reporting and data system (TIRADS) has been developed. This study aims at evaluating the reliability of the daily use of ACR-TIRADS classification in differentiating between benign and malignant thyroid lesions and hence avoiding invasive FNAC procedure in benign lesions. 60 patients , patients with clinically suspected or ultrasound diagnosed thyroid nodules were subjected to undergo conventional high resolution sonography of the thyroid gland and then categorized according to ACR-TIRADS Classification. Ultrasound guided fine needle aspiration cytology (FNAC) of suspicious or dominant nodule were evaluated according to BETHESDA system of Classification for reporting thyroid cytopathology. The relation between TIRADS and BETHESDA was evaluated using the histopathological report , which was used as the standard final diagnosis for Comparison. In this study on comparing the TIRADS scoring system on initial assessment to predict the efficacy in differentiating benign and malignant lesions versus the final biopsy report it is found that TIRADS is effective in predicting malignancy in a suspicious thyroid nodule with significant p value of 0.05 and with sensitivity of 77.36 % specificity of 57.14% positive predictive value (PPV) of 93.18% and negative predictive value of (NPV) 25% at 95% confident interval. Aims and
2. Journal of Cell and Tissue Research Vol. 23(2): 7295-7301 (2023)
Heavy metal intoxication, mobilization and therapy in children and adults: A review
Sood, P. P. and Tyagi, S.
Former Emeritus Medical Scientist, ICMR , New Delhi, Emeritus UGC Scientist, New Delhi, Professor
and Head, Department of Biosciences, Saurshtra University, Rajkot 360 005 (Gujarat).
Abstract: The survival of human race depends upon, better environmental management. Therefore, continuous and sincere efforts will have to be carried out by everyone involved in environmental management, protection, monitoring, assessment, research, education, planning, conservation and sustainable development to use the resources. ATSDR toxicological profiles, published in USA have reported a number of hazardous chemicals and characterized the toxicological and adverse health effects. In children heavy metals effect respiratory disorders, cancer, cardiovascular and neurological diseases. Several heavy metals lnteract pro-oxidatively with the phagocytic cells of the innate immune system. Nevertheless, these as well as other recent studies are incapable to provide much information about their total removal from body. Therefore, studies on this subject are regularly carried out by several investigators where the animals were intoxicated with hazardous heavy metals, such as mercury, methyl mercury, lead, chromium, cadmium, aluminium, zink, and other toxicants such as floride and arsanic etc. and thereafter, pre- or post therapies were provided to eliminate the toxicants, to improve their health and to restore the altered conditions caused thereby. The objective of present contribution is to review the toxic effect of some heavy metals and possible therapy provided with antioxidants.
Keywords: Heavy metals, Intoxication, mobilization , Therapy
3. Journal of Cell and Tissue Research Vol. 23(2): 7303-7310 (2023)
Phytochemical screening, free radical scavenging activity, invitro antioxidant and antihelminthic activity of mucuna pruriens seed
Chitra Kalyanaraman
1Dr. ALM Post Graduate Institute of Basic Medical Sciences, Taramani, Chennai, TamilNadu, India.
Abstract: Objective: Mucuna pruriens (M. pruriens) is a legume plant that has been used in traditional medicine for its various therapeutic benefits. It is one of the essential ingredients in herbal medication used to treat a variety of illnesses, including male sexual dysfunction. In this study phytochemical capabilities, In-vitro antioxidant capacity, and antihelminthic activity of methanolic extract of M. pruriens seeds were investigated.The preliminary phytochemical screening of M. pruriens was carried to find out the active constituents responsible for the pharmacological activities of the seed extract.The antioxidant activity of the M. pruriens were performed by using the (2,2 -Diphenyl -1- picrylhydrazyl) DPPH radical scavenging assay and Nitric oxide radical scavenging assay at different concentration compared with standard Vitamin C. Also, the seed extract was tested for its antihlementhic activity against two intestinal nematodes, Ascaridia galli( A. galli) and Cestode parasites Raillientina tetragona (R. tetragona).The present study supports that M. pruriens contains high level of antioxidant such as phenols, flavonoids and alkaloids which have been found to scavenge free radical and reduce oxidative stress in body. M. pruriens seed extract exhibits 50% more inhibition activity at the concentration of 330μg/ml. The results also shows that M. pruriens seed extract that contains alkaloids and tannins, which have been shown to possess antihlementhic activity against intestinal worms and parasites.In conclusion, methanolic extract of M. pruriens exhibits antioxidant and antihelminthic property which could be used to develop new drugs for the treatment of oxidative stress-related diseases and parasitic infections on humans.
Keywords: Antihelminthic , Mucuna pruriens seed.
4. Journal of Cell and Tissue Research Vol. 23(2): 7311-7314 (2023)
Effect of dietary chromium supplementation in broiler diet on serum lipid profile and abdominal fat percentage
Sathyabama, T. and Kirubakaran, A.
Department of Veterinary Physiology, Veterinary College and Research Institute, Namakkal-637002.
E-mail: drtsbama@gmail.com
Abstract: The present study was conducted to compare the serum lipid profile and abdominal fat percentage in 360 numbers of day old broiler chicks divided as six groups fed three sources of chromium. T1 group serve as control fed standard diet without chromium (Cr) supplementation. Inorganic chromium chloride was fed to group 2, organic chromium in two forms - Chromium propionate CrProp (T3), organic Chromium picolinate CrPic (T4), at 200 µg and nano chromium (nanoCr) source at two dosage levels of 100 and 200 µg chromium per kg broiler diet was given to T5 and T6 groups respectively. The serum lipid profile and abdominal fat weight at the end of sixth week of the trail was recorded. The observed per cent decrease in Low density lipoprotein cholesterol (LDL-C), triglycerides, abdominal fat and increase in High density lipoprotein cholesterol (HDL-C) levels were 35.724, 24.033, 28.261 and 22.154 respectively in nanoCr 200 µg/kg fed T6 group. The results indicated that dietary nanoCr followed by organic CrPic supplementation had significantly (p<0.05) decreased the LDL-C, triglycerides and abdominal fat and HDL-C level was increased at 200 µg of nanoCr/kg without any significant change in total cholesterol level when compared to other groups. Chromium could improve lipoprotein lipase and lecithin cholesterol acyltransferase activity which in turn accelerated cholesterol esterification and excretion, promoting the synthesis of HDL-C thereby increasing LDL-C uptake by hepatocytes and reducing the blood LDL-C content.
Keywords: Inorganic chromium, Lipid profile, Abdominal fat, Broiler.
5. Journal of Cell and Tissue Research Vol. 23(2): 7315-7320 (2023)
Clinic alimplications of pancreatic cancers in immune associated therapies
Kumaresan, H.
School of Infection Immunity, University of Glasgow, Glasgow G128QQ. United Kingdom.
E. mail: harinikumaresan@yahoo.com, Cell: +44-7493512646
Abstract: Pancreatic ductal adenocarcinoma (PDAC) is regarded as a devastating disease with poor prognosis. Late detection, aggressive pathogenesis, immune deserting of tumor and surgical restrictions are major challenges that limit therapeutic interventions. In this review, we discuss stratification, immune biomarkers, their analytical assessments, and clinical implications in therapeutic fortes according to patient suitabilites. On those lines, we also highlight the significant drawbacks in clinical interventions that could lead to potential research scopes such as increasing drug efficacy, extending patient survival and quality of life. Additionally, we have also discussed the genomic and immunological attributes of pancreatic cancer for better understanding and development of potential treatment approaches, focusing mainly on immunotherapies.
Keywords: PDAC, Adenocarcinoma, Immune biomarkers, Immunotherapy.
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6. Journal of Cell and Tissue Research Vol. 23(2): 7321-7325 (2023)
Faecal transplant technology in therapeutics of Alzheimer’s
Pushkala, K.
Former, Associate Professor, S. D. N. B. Vaishnav College for Women, Chennai, India. E. mail: kpushkala@gmail.com,
Abstract: In the mid-1900s, at the time when medicine became science and found treatments for many diseases, nevertheless many are not under control, especially brain disorders. A nerve injury can affect the brain’s ability to communicate with muscles and organs. Early diagnosis and treatment may prevent complications and permanent damage. Faecal Transplant Technology (FMT), a newly developed and effective therapeutic method for many diseases also showed promise to manage some of the brain disorders. Here the progress report of the application of FMT in the management and possible treatment of Alzheimer’s disease is discussed.
Keywords: Alzheimer’s, FMT, Gut microbiota
7. Journal of Cell and Tissue Research Vol. 23(2): 7327-7334 (2023)
An overview of the increasing landscape of artificial intelligence in healthcare and medicine use cases
Gupta, M. and Bhargava, R.
37740 Spring Tide Rd, Newark, CA 94560, United States. E.mail: mridulg@alumni.cmu.edu;
phone:1-(412) 519-8153), E. mail: bhargavr@purdue.edu;
Abstract: Artificial intelligence (AI) has emerged as a transformative technology with the potential to revolutionize healthcare. This paper explores the various applications of AI in healthcare, including disease diagnosis, medical imaging, drug discovery, personalized medicine, and healthcare management. The paper discusses the benefits, challenges, and future directions of AI in healthcare, highlighting the potential to enhance patient outcomes, improve efficiency, and advance medical research. Through a survey of recent studies and industry reports, this paper provides valuable insights into the current state and future prospects of AI in healthcare.
Keyword: Artificial intelligence, healthcare.
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8. Journal of Cell and Tissue Research Vol. 23(2): 7337-7355 (2023)
Potato (Solanum tuberosum.) as a rich source of nutrients and bioactive compounds: A review
Piotr Barbas, Ali Hulail Noaema and Barbara Sawicka*
*Department of Plant Production Technology and Commodities Science, University of Life Science in Lublin, Akademicka 15 str., 20-950 Lublin, Poland, E. mail: barbara.sawicka@up.lublin.pl
Abstract: The potato is the basis of the human diet in most countries of the world and has a significant impact on human health. Therefore, the aim of the work was to draw attention to the potato as a source of nutrients and energy as well as bioactive compounds and to use them in human and animal nutrition, in pharmacy, medicine and cosmetology. A quantitative analysis of the scientific literature indicating the beneficial effect of potato in the daily diet was carried out. The search for nutrients and bioactive compounds was based on the Scopus database. Potato tubers have a strong prebiotic effect, as they lower the pH in the intestinal environment and have an immunomodulatory effect. These properties of potato are the basis for its use in the treatment of many diseases such as diabetes, obesity, hypertension, diseases of the nervous system and cancer. In addition, potato normalizes lipid metabolism disorders by lowering elevated cholesterol levels; is helpful in the treatment of type II diabetes by reducing elevated glucose levels; facilitates slimming processes (reduces body weight) and lowers the level of uric acid. Potato also has an immunostimulant effect, improving metabolism (in disorders of lipid metabolism), while in diseases of the cardiovascular system it regulates heart rhythm disorders, and is also used in limitingthe development of some malignant types of cancer. Prevents many chronic infectious diseases; chronic fatigue states; disorders of the intestinal microflora and the immune system.
Keywords: Potato, Nutrients, Bioactive Compounds
9. Journal of Cell and Tissue Research Vol. 23(2): 7357-7360 (2023)
Food and health : past, present and future: A review
Asha, A. K.
Department of Veterinary Physiology and Biochemistry, Veterinary College and Research
Institute, Udumalpet.Cell: ashahsn@gmail.com, Cell: 9486707385
Abstract: Food is one of the basic necessities of life. Food contains nutrients—substances essential for the growth, repair, and maintenance of body tissues and for the regulation of vital processes. Nutrients provide the energy our bodies need to function. The energy in food is measured in units called calories. In this review sources of food,its preservation for future use and various food practices were reviewed.
Keyword: Food, Diet, Health
10. Journal of Cell and Tissue Research Vol. 23(2): 7361-7366 (2023)
The interplay between neurodegeneration, neuroinflammation, gut microbiota, and the blood-brain barrier: elucidating their interconnected relationship
Akila Parvathy Dharshini. S.
School of medicine, Stanford University, USA. E. mail: akilabioinfo@gmail.com
Abstract: Neurodegenerative diseases are complex disorders that pose significant challenges to global health. Recent research has shed light on the intricate relationship between neurodegeneration, neuroinflammation, gut microbiota, and the blood-brain barrier (BBB). This article provides a comprehensive review of the current knowledge on the interplay between these factors and highlights the key findings from relevant studies. Understanding the complex interactions among neurodegeneration, neuroinflammation, gut microbiota, and the BBB will pave the way for novel therapeutic approaches targeting multiple aspects of these debilitating diseases.
Keyword: Neurodegeneration, Neuroinflammation, Gut Microbiota, BBB
11. Journal of Cell and Tissue Research Vol. 23(2): 7367-7370 (2023)
Antibodies in neurological disorders
Gupta, P.,1 Swadha Das Guru2, Gupta, S. K.3
1Wayne State University – Detroit Medical Center, Detroit, Michigan, 2Henry Ford Hospital,
Detroit, Michigan, 3Eternal Hospital, Jaipur, India E. mail: drguptasuresh@gmail.com
Abstract: Detection of specific autoantibodies to neural targets has resulted in a better understanding of autoimmune neurological disorders. Neural antibodies have led to categorization of neurological diseases disease as autoimmune which were previously thought to result from infectiousor psychogenic causes or considered idiopathic. Antibodies directed to intracellular antigens are commonly associated with cancer and are poorly responsive to immunotherapy. Cell surface antibodies on the other hand are directly pathogenic and mediate the neurological disease. This review summarizes the neurological disorders, antigenic targets and pathogenic mechanisms of most common neural antibodies.
Keywords: Neurological disorders
12. Herbs and herbal extracts, the future medicines (problems and solutions): A review
Badami, S.
SDM College of Pharmaceutical Sciences, SDM University, Dharwad-580009, Karnataka, India. Email: shribadami@rediffmail.com
Abstract: Herbal drug and Phyto chemists were so far involved in research unknowingly utilizing not so perfect methodologies, means not considering the effects of temperature during powdering and extraction, selecting solvents other than those used in traditional medicine and testing the plants for their pharmacological activities in a manner different from traditional use. Only on experience, one will accumulate knowledge and solve such errors. In this article, some such ideal suggestions for medicinal plant researchers is given in order to protect the traditional knowledge in the hands of untrained wrong testing methodology followers.
Keywords: Herbal medicine
13. Journal of Cell and Tissue Research Vol. 23(2): 7375-7384 (2023)
OLIG2-astrocytes: a comprehensive mini-review of a distinct astrocytic subpopulation
Tatsumi, K. and Wanaka, A.
Department of Anatomy and Neuroscience, Nara Medical University, Faculty of Medicine, Nara Medical University, 840 Shijo-cho, Kashihara City, Nara 634-8521, Japan. E.. mail: radha815@naramed-u.ac.jp,
Abstract: Astrocytes, the most populous glial cell type within the central nervous system (CNS), are recognized to form diverse cohorts characterized by unique morphologies, gene expressions, and functionalities. Moreover, these cells are allocated to geographically discrete domain regions throughout development. Such regional heterogeneity implies a significant role of astrocytes in modulating the postnatal establishment of neural circuits, thereby orchestrating the ultimate neuronal activity. In our lineage tracing investigation of Olig2-expressing cells utilizing Olig2CreER; EYFP (or tdTomato) transgenic mice, we unearthed Olig2 lineage mature astrocytes within the adult forebrain. Adequate recombination induction was accomplished via prolonged administration of tamoxifen, unveiling a predilection of Olig2 lineage astrocytes to amass in specific adult brain nuclei. Intriguingly, these nuclei predominantly receive GABA inputs. Concurrently, in these regions, the expression of glial fibrillary acidic protein (GFAP), a principal cytological indicator of astrocytes, was remarkably faint, and a unique localization was observed with GFAP-positive astrocytes. In essence, Olig2 lineage mature astrocytes appear to constitute a distinct subpopulation, exhibiting a differential brain distribution and separate functionalities from GFAP-positive astrocytes. This manuscript presents a synthesis of our current understanding regarding the Olig2 lineage mature astrocyte subpopulation that we have discerned.
Keywords: Olig2-astrocyte, GFAP-astrocyte, inhibitory synapse, GAT-3, slc7a10
14. Journal of Cell and Tissue Research Vol. 23(2): 7385-7388 (2023)
Regulatory considerations for biotherapeutics: focus on chemistry, manufacturing and controls:
A review
Gupta, A.
Senior Associate Director, Global Regulatory Affairs, Boehringer Ingelheim Pharmaceuticals Inc.,
Ridgefield, CT, USA. E.mail: alpana_gupta@hotmail.com
Abstract: Biotherapeutics, also known as biologics, are therapeutic agents derived from living organisms. They include a wide range of products such as monoclonal antibodies, recombinant proteins, gene and cell therapies. Due to their complex nature and unique manufacturing processes, biotherapeutics have specific regulatory requirements to ensure their safety, efficacy, and product quality. The production of biotherapeutics is a complex and highly regulated process that requires a deep understanding of molecular biology, cell culture, and protein chemistry. As the demand for these life-saving therapies continues to grow, ongoing research and innovation in the field of biotherapeutics production will be essential to ensure the development of safe, effective, and affordable treatments for a wide range of indications,including cancer, autoimmune disorders, and infectious diseases. The health authorities in the major regions have guidelines and regulations in place to ensure that the manufacture of biologic drugs is in compliance with Good Manufacturing Practices (GMP), and appropriate measures are in place for the chemistry, manufacturing and controls (CMC) of these therapeutics.Regulatory CMC considerations play a crucial role in the development, manufacturing, and approval of biotherapeutics. By understanding and addressing these considerations, companies can ensure the quality of their biotherapeutic products, ultimately benefiting patients and healthcare providers worldwide.As the field of biotherapeutics continues to advance, it is essential for regulatory agencies and developers to collaborate and adapt to the unique challenges and opportunities presented by these groundbreaking therapies.
Keywords: Biotherapeutics
15. Journal of Cell and Tissue Research Vol. 23(2): 7389-7396 (2023)
Biocomposition of dental implants. A review
Wang, S. and Gupta, G.
1Department of Microbiology and Molecular Genetics, University of California at Davis, Davis CA 95616. E. mail: swang52@gmail.com: 2University of Pennsylvania School of Dental Medicine, Philadelphia PA 19104.
E. mail: gg718104@gmail.com
Abstract: Dental implants are mechanical devices that are designed to substitute individual missing teeth and are the best existing remedy for tooth loss. Modern dental implant design involves devices composed of three different parts—the crown, the abutment, and the implant fixture. The materials and surface treatments used to design the different parts of a dental implant are carefully selected for their functions. The biomaterials selected to construct the crown must be biologically inert to withstand degradation due to acidic foods and aesthetically resemblant of the natural tooth. Alternately, the biomaterials used for the implant fixture target strength, durability, and assimilation into surrounding biological tissues. The implant fixture, or the “artificial tooth root,” is the most critical component in terms of biocompatibility because it directly interacts with the jawbone. The implant fixture achieves direct bone to implant contact through osseointegration, which is a key process permitting the biological compatibility of the implant within the body. This paper aims to discuss currently existing dental implants and their relevant features in relation to their use as a biocompatible tooth replacement.
Keywords: Dental implants
16. Journal of Cell and Tissue Research Vol. 23(2): 7397-7402 (2023)
Wound healing: A short review
Prathiba, V..
Former Scientist, Central Leather Research Institute, Chennai E. mail: v_prathiba@hotmail.com , Cell: 9444673986
Abstrct: Wound healing is a complex process and it is the result of cell-cell interactions and cell-matrix interactions. The different phases of wound healing are controlled by these interactions and growth factors that are released in phase specific manner. In certain predisposed individuals, these processes are not regulated in phase specific manner due to lack of timely signals and normal process of wound healing is disturbed which may result into scar formation such as hypertrophic scars and keloids. The factors involved in abnormal wound healing are addressed in the present review.
Key words: wound healing,
17. Journal of Cell and Tissue Research Vol. 23(2): 7403-7408 (2023)
Multilayering of central zone lens epithelial cells in human cataracts
Kaid Johar, S. R, Tatsumi, K. and Vasavada, A.
Iladevi Cataract & IOL Research Centre, Ahmedabad, India; Department of Anatomy II,
Nara Medical University, Nara, Japan
Abstract: The lenticular epithelium (LE) is arranged in monolayer at the anterior surface of the lens below lens capsule. Due to stress, the lens epithelial cells (LECs) extrude out of the monolayer, get superimposed and become multilayered. In the present study, we have detected the number of superimposed cells in different types of cataracts using flat preparations along with the study of semithin sections and immunofluorescence labeling of proliferative cells by labeling topoisomerase II. LE obtained from clear cadaver lenses served as a control. Superimposition of cells was not found in the capsulorhexis samples obtained from clear lenses and nuclear cataracts while it was highest in the posterior subcapsular cataract followed by cortical and mix type of cataracts. Semithin section studies indicate the relationship of the superimposed cells with the surrounding cells. The number of proliferative cells was highest in the posterior subcapsular cataract followed by cortical cataract, mix cataract, clear lenses and nuclear cataract. Superimposition of LECs is found in posterior subcapsular, cortical and mix types of cataracts and is associated with higher proliferation.
Keywords: Multilayering of the lens epithelium, Human cataracts
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18. Journal of Cell and Tissue Research Vol. 23(2): 7409-7412 (2023)
Seasonal variations in oocyte and embryo retrieval during in vitro fertilization: A review
Rasool, A. and Sarath, T.
Department of Veterinary Gynaecology and Obstetrics, Tamil Nadu Veterinary and Animal Sciences
University, Chennai, India. E. mail: mirakhter1312@gmail.com
Abstract: In recent years, in vitro fertilization (IVF) and its associated technologies have demonstrated remarkable advancements. This assisted reproductive technique holds the potential to achieve a greater number of oocytes or embryos collected from donor and higher conception rates. Utilizing elite female donors for oocyte retrieval can also facilitate the production of offspring of the desired sex through IVF. In humans, this technique can be employed to produce offspring in individuals with low fertility or reproductive tract diseases. However, several factors have been identified that can potentially diminish the overall conception rate. These factors encompass reduced fertility, advanced maternal age, nutritional considerations, seasonal variations, and compromised oocyte or embryo quality. This review aims to elucidate the potential influence of external factors, specifically seasonal variation, on oocyte and embryo recovery rates.
Keywords: In vitro fertilization, Pregnancy rate, Oocyte, Embryo quality, Seasonal effect.
19. Journal of Cell and Tissue Research Vol. 23(2): 7413-7420 (2023)
Association of Zip14 gene polymorphism in colorectal cancer of Telangana & Andhra Pradesh Cohort
Beesa, S., Bodiga, S., Sowjanya, P., Avancha, R., Latha, J. and Bodiga, V. L.
Department of Molecular Biology, Institute of Genetics and Hospital for Genetic Diseases (Osmania University), Begumpet, Hyderabad, Telangana. India-500016;E. mail: suneethabeesa07@gmail.com,
Abstract: Zinc is one of the essential trace elements and is involved in various functions in the body. Zinc dyshomeostasis is caused by the dysfunction of zinc transporters can contribute to the initiation or progression of various cancers including Colon cancer. Incidence rates of Colorectal cancers (CRC) are on high rise in developing countries like India. ZIP14, one of the Zinc transporter plays a major role in inflammation, a hallmark of Colorectal cancer. The mechanism involved in the role of ZIP14 in CRC is not fully understood but, single nucleotide polymorphisms (SNPs) can be used to predict its risk and prognosis. In the present case- control study, we investigated the relationship between ZIP14 SNP rs1051708 A>C, (Chromosome location 8p21.3), and the risk of CRC. The frequency of the polymorphism in the case and control groups was determined using the PCR-RFLP method. The association of age, gender, diet, and smokeless tobacco to CRC was calculated with different statistical parameters. The ZIP14 A>C rs1051708 variation was found to be significantly associated with CRC. However, the frequency of, the C allele independently and in the heterozygote state was significant in CRC association. Diet and usage of smokeless tobacco showed a significant association with CRC in subjects below 50 years of age. It is concluded that the occurrence ‘C’ allele of ZIP14 (rs1051708) SNP in genotype and Diet, and Tobacco consumption in adults below 50years are associated with CRC susceptibility alarming better awareness and diagnostic modalities leading to the detection of colon cancer among adults below 50 years given increasing incidence in India
Keywords: Zinc, Zip14, Polymorphism, PCR-RFLP
20. Journal of Cell and Tissue Research Vol. 23(2): 7421-7427 (2023)
Phytochemical analysis of Pterocarpus marsupium and Swertia chirayita for their secondary metabolites having antidiabetic properties by HPTLC
Manjunatha, K. P.,* Sathyanarayana, M. L. and Shesharao
Assistant Professor Department of Veterinary Pathology, Veterinary College, Hebbal, KVAFSU, Bangaluru. E.mail: ahpdornalli@kvafsu.edu.in , Cell: 8618111083:
Abstract: Pterocarpus marsupium and Swertia chirayita are known for their hypoglycemic and antidiabetic properties in Ayurvedic system of medicine. The effects of these plants may delay the development of diabetic complications and correct the metabolic abnormalities. Secondary metabolites are responsible for medicinal activity of these plants. Qualitative phytochemical analysis of these plants confirms the presence of various phytochemicals mainly Antherecene derivatives, Bitter principles, Flavinoids, Coumarins and Glycosides. The results suggest that the phytochemical property of these plants which having bioactive constituents are responsible for their hypoglycemic and antidiabetic effects.
Keywords: Pterocarpus marsupium, Swertia chirayita, antidiabetic
.21. Journal of Cell and Tissue Research Vol. 23(2): 7429-7436 (2023)
Pathomorphological and immunohistochemical evaluation of Pterocarpus marsupium and Swertia chirayita herbal extractfor their antidiabetic effect in diabetes induced wister rats
Manjunatha, K. P., Sathyanarayana, M. L. and Shesharao
Assistant Professor Department of Veterinary Pathology, Veterinary College, Hebbal, KVAFSU, Bangaluru. E.mail: ahpdornalli@kvafsu.edu.in , Cell: 8618111083:
Abstract:The present study was undertaken to evaluate the efficacy of Pterocarpus marsupium and Swertia chirayita individualy and in combination along with glibenclamide in streptozotocin induced diabetic rats for a period of 45 days. There was significant variation in different groups in term of Pathomorphological and Immunohistochemical findings of diabetic rats when compared to normal control rats. The alleviation of the diabetes and its complications induced by streptozotocin was observed in all the treatment groups with variable degrees of improvement. Pterocarpus marsupium and Swertia chirayita extracts were effective in alleviating streptozotocin induced diabetes and were comparable with glibenclamide. Combination of Pterocarpus marsupium with glibenclamide and Swertia chirayita with glibenclamide showed better improvement compared to individual extracts alone but statistically no significant synergetic action was seen. Synergistic effect was observed in combination of Pterocarpus marsupium and Swertia chirayita and in combination of Pterocarpus marsupium and Swertia chirayita along with glibenclamide.
Keywords: Pterocarpus marsupium, Swertia chirayita, Diabetes, Glibenclamide, Streptozotocin
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